陳皓君 教授　Chen, Hauh-Jyun Candy                 Professor

Date of employment: Aug. 1, 1997

Specialty: Bio-Organic,

     Bio-Analytical Chemistry

TEL (O): (886-5) 272-0411

     ext. 66403

Email:chehjc@ccu.edu.tw

Lab Webpage

到校日期：86年8月1日

專長領域：生物分析化學 、質譜分析、生物化學、毒物化學

聯絡電話：(05) 272-0411 ext. 66403

電子信箱：chehjc@ccu.edu.tw

研究室：Rm 408

實驗室網頁

B.S. in Chemistry, National Cheng Kung Univ., 1983)

Ph.D. in Chemistry, State University of New York at Stony Brook, 1988

Postdoc Fellow, NIH, 1988-1989

Postdoc Assoc., Rockefeller Univ., 1989-1991

Senior Research Fellow, American Health Foundation, 1992-1994

Associate Research Scientist (tenure-track), American Health Foundation, 1994-1997

Assistant Prof., National Chung Cheng Univ., 1997-1999

Associate Prof., National Chung Cheng Univ., 1999-2004

Professor, National Chung Cheng Univ., 2004-

學士 國立成功大學化學系理學士 (1983)

博士 美國紐約州大石溪分校有機化學博士 (1988)

美國國家衛生總署博士後研究員 (1988-1989)

美國洛克婓勒大學博士後研究員 (1989-1991)

美國健康基金會資深研究員 (1992-1994)

美國健康基金會副研究科學家 (1994-1997)

國立中正大學化學系助理教授 (1997-1999)

國立中正大學化學系副教授 (1999-2002)

國立中正大學化生系副教授 (2002-2004)

國立中正大學化生系教授 (2004-)

Research Interests

DNA and Protein Adducts Derived From Endogenously Generated Reactive Species

Effect of Biological and Dietary Antioxidants in Inhibiting DNA and Protein Adducts Formation

Analysis of DNA and Protein Adducts as Biomarkers for Cancer Risk Assessment Using GC-MS and LC-MS/MS

Study of Post-Translational Modifications of Proteins by LC-MS/MS-Based Proteomic Approach

研究重點

生物體內產生之具反應性物種與DNA或蛋白質加成產物之研究

食物中與生物體內之抗氧化劑能否降低DNA或蛋白質加成產物之研究

以GC-MS或LC-MS/MS偵測體內DNA加成產物作為患癌症危險性評估的生化指標之研究

以LC-MS/MS之蛋白質體學法研究蛋白質轉譯後修飾之結構與功能之改變

Selected Publications 代表著作：

• Chen, H.-J. C.,* and Liu, Y.-F. (2013) “Simultaneous quantitative analysis of N³-ethyladenine and N⁷-ethylguanine in human leukocyte DNA by stable isotope dilution capillary liquid chromatography nanospray ionization tandem mass spectrometry.” J. Chromatogr. A. 1271(1), 86–94. (Impact Factor: 4.531)

• Chen, H.-J. C.,* and Chen, Y.-C. (2012) “Reactive nitrogen oxide species­-induced post-translational modifications in human hemoglobin and the association with cigarette smoking.” Anal. Chem. 84(18), 7881-7890. 

• Chen, H.-J. C.,* Wang, Y.-C., and Lin, W.-P. (2012) “Analysis of ethylated thymidine adducts in human leukocyte DNA by stable isotope dilution nanoflow liquid chromatography nanospray ionization tandem mass spectrometry.” Anal. Chem. 84, 2521–2527.

• Chen, H.-J. C.,* Lin, W.-P. (2011) “Quantitative analysis of multiple exocyclic DNA adducts in human salivary DNA by stable isotope dilution nanoflow liquid chromatography nanospray ionization tandem mass spectrometry.” Anal. Chem. 83(22), 8543-8551. (Impact Factor: 5.874)

• Chen, H.-J. C.* (2011) “Analysis of DNA adducts in human samples: Acrolein-derived exocyclic DNA adducts as an example.” Mol. Nutr. Food Res. 55(9), 1391-1400. (IF: 4.713) (invited review article)

• Chen, H.-J. C.,* Lin, G.-J., and Lin, W.-P. (2010) “Simultaneous quantification of three lipid peroxidation-derived etheno adducts in DNA by stable isotope dilution nanoflow liquid chromatography nanospray ionization tandem mass spectrometry.” Anal. Chem. 82, 4486–4493. (Impact Factor: 5.712)

• Chen, H.-J. C.,* and Lin, W.-P. (2009) “Simultaneous quantification of 1,N²-Propano-2¢-deoxy­guanosine adducts derived from acrolein and crotonaldehyde in human placenta and leukocytes by isotope dilution nanoflow LC nanospray ionization tandem mass spectrometry.” Anal. Chem. 81(23), 9812–9818. (Impact Factor: 5.712)

• Chen, H.-J. C.,* and Chen, Y.-C. (2009) “Analysis of glyoxal-induced DNA cross-links by capillary liquid chromatography nanospray ionization tandem mass spectrometry” Chem. Res. Toxicol. 22(7), 1334-1341. (Impact Factor: 3.491)

• Chen, H.-J. C.,* and Chiu, W.-L. (2008) “Simultaneous detection and quantification of 3-nitrotyrosine and 3-bromotyrosine in human urine by stable isotope dilution liquid chromatography tandem mass spectrometry” Toxicol. Lett. 181(1), 31-39. (Impact Factor: 3.249)

• Chen, H.-J. C.,* Chiu, W.-L., Lin, W.-P., and Yang, S.-S. (2008) “Investigation of DNA-protein cross-links formation between lysozyme and oxanine by mass spectrometry” ChemBioChem, 9(7), 1074-1081. (Impact Factor: 4.100)

• Chen, H.-J. C.,* Chang, C.-M., Lin, W.-P., Cheng, D.-L., and Leong, M.-I. (2008) “H₂O₂/Nitrite-induced posttranslational modifications of human hemoglobin determined by mass spectrometry: Redox regulation of tyrosine nitration and 3-nitrotyrosine reduction by antioxidants” ChemBioChem, 9(2), 312-323. (Impact Factor: 4.100)

• 陳育進、邱偉倫、陳皓君* (2007) “以質譜法量測與抽煙有關之DNA加成產物作為生化指標 (Measurement of Smoking-Related DNA Adducts as Biomarkers by Mass Spectrometry.)”CHEMISTRY, 65(2), 193-210.

• Chen, H.-J. C.,* Hsieh, C.-J., Shen, L.-C., and Chang, C.-M. (2007) “Characterization of DNA-protein cross-links induced by oxanine, a cellular damage derived from nitric oxide and nitrous acid” Biochemistry 46(13), 3952 – 3965. (Impact Factor: 3.633)

• Chen, H.-J. C.,* and Kao, C.-F. (2007) “Effect of gender and cigarette smoking on urinary excretion of etheno DNA adducts in humans measured by isotope dilution gas chromatography/mass spectrometry” Toxicol. Lett. 169(1), 72-81. (Impact Factor: 2.784)

• Tsai, M. (蔡旻都), and Chen, H.-J. C.* (陳皓君) (2006) “蔬果中類黃酮之抗氧化作用與生物活性(Antioxidative and biological activity of flavonoids in fruits and vegetables)” CHEMISTRY, 64(3), 301-315. (Review in Chinese) (the cover artwork article)

• Chen, H.-J. C.,* and Chiu, W.-L. (2005) “Association between cigarette smoking and urinary excretion of 1,N²-ethenoguanine measured by isotope dilution liquid chromatography/­electrospray ionization-tandem mass spectrometry” Chem. Res. Toxicol. 18(10), 1593-1599. (the cover artwork article) (Impact Factor: 3.508) (23%, #8/35 in Chemistry, Medicinal)

• Chen, H.-J. C.,* Wu, C.-F., and Huang, J.-L. (2005) “Measurement of urinary excretion of 5-hydroxymethyluracil in human by GC/NICI/MS: Correlation with cigarette smoking, urinary TBARS and etheno DNA adduct.” Toxicol. Lett. 155(3), 403-1410. (Impact Factor: 2.784)

• Chen, H.-J. C.,* and Chang, C.-M. (2004) “Quantification of urinary excretion of 1,N⁶-ethenoadenine, a potential biomarker of lipid peroxidation, in humans by stable isotope dilution liquid chromatography/electrospray ionization-tandem mass spectrometry: Comparison with gas chromatography-mass spectrometry.” Chem. Res. Toxicol. 17(7), 963-971. (SCI) (Impact Factor: 3.332)

• Chen, H.-J. C.,* Wu, C.-F., Hong, C.-L., and Chang, C.-M. (2004) “Urinary excretion of 3,N⁴-etheno-2'-deoxycytidine as a biomarker of oxidative stress: Association with cigarette smoking.” Chem. Res. Toxicol. 17(7), 896–903. (SCI) (Impact Factor: 3.332)

• Chen, H.-J. C.* (2004) “Formation, analysis and repair of exocyclic etheno DNA adducts” Chin. Pharm. J. 56(1), 1-16. (Invited review in English)

• Chen, H.-J. C.* (2003) “液相層析質譜儀於致癌風險評估上之應用：去氧核糖核酸加成產物之分析 (Applications of Liquid Chromatography/Mass Spectrometry in Cancer Risk Assessment: Analysis of DNA Adducts)”科儀新知 Instruments Today 25(3), 47-54. (invited article)

• Chen, H.-J. C.,* Hong, C.-L., Wu, C.-F., and Chiu, W.-L. (2003) “Effect of cigarette smoking on urinary 3,N⁴-ethenocytosine levels measured by gas chromatography/mass spectrometry.” Toxicol. Sci. 76(2), 321-327. (SCI) (Impact Factor: 3.367)

• Chen, H.-J. C.,* and Chiu, W.-L. (2003) “Detection and quantification of 1,N⁶-ethenoadenine in human urine by stable isotope dilution capillary gas chromatography/negative ion chemical ionization/mass spectrometry.” Chem. Res. Toxicol. 16(9), 1099-1106. (SCI) (Impact Factor: 3.607)

• Chen, H.-J. C., Wu, S.-B., and Chang, C.-M. (2003)“Biological and dietary antioxidants protect against DNA nitration induced by reaction of hypochlorous acid with nitrite.” Arch. Biochem. Biophys. 415(1), 109-116. (SCI) (Impact Factor: 2.606)  

• Chen, H.-J. C.; Chang, C.-M,; Chen, Y.-M. (2003) "Hemoprotein-Mediated Reduction of Nitrated DNA Bases in the Presence of Reducing Agents." Free Radic. Biol. Med. 34(2), 254-268.(Impact Factor: 5.533)  

• Tsai, C.-C.; Chen, H.-J. C. (2002)“生物體內梅納反應的化學與其抑制 (Chemistry of the Maillard reaction and its inhibition in vivo.)” CHEMISTRY, 60(3), 335-349. (Review in Chinese) (Supported by NSC 90-2113-M-194-023)

 

• Lee, S.-Y., and Chen, H.-J. C. (2002)“過氧亞硝酸根負離子與去氧核醣核酸的化學反應及其生物效應 (Peroxynitrite-induced DNA damage and its biological consequences.)”CHEMISTRY, 60(3), 319-334. (Review in Chinese)

(Supported by NSC 90-2113-M-194-023)

• Chen, H.-J. C. (2002)“質譜在分析 DNA 加成產物之重要性與其在毒物學上之意義(Analysis of DNA adducts by mass spectrometry: Importance and implication in toxicology.)” CHEMISTRY, 60(2), 189-209. (Invited review in Chinese)  

• Chen, H.-J. C.; Chen, Y.-M.; Chang, C.-M. (2002) "Lipoyl dehydrogenase catalyzes reduction of nitrated DNA and protein adducts using dihydrolipoic acid or ubiquinol as the cofactor." Chem.-Biol. Interact. 140(3), 199-213. (SCI) (Impact Factor: 1.261)  

• Chen, H.-J. C.; Wang, T.-F.; Chen, Y.-M. (2002) "Role of nitrite on nitration of 2'-deoxyguanosine by nitryl chloride." J. Chin. Chem. Soc. 49(2), 275-281. (SCI) (Impact Factor: 0.525)  

• Chen, H.-J. C.; Row, S.-W.; Hong, C.-L. (2002) "Detection and quantification of 5-chlorocytosine in DNA by isotope dilution gas chromatography/negative ion chemical ionization/mass spectrometry." Chem. Res. Toxicol. 15(2), 262-268.

 

• Chen, H.-J. C., Lin, T.-C., Hong, C.-L., and Chiang, L.-C. (2001) "Analysis of 3,N⁴-ethenocytosine in DNA and in human urine by isotope dilution gas chromatography/negative ion chemical ionization/mass spectrometry." Chem. Res. Toxicol. 14(12), 1612-1619

• Chen, H.-J. C., and Hong, C.-L. (2001) "Endogenous DNA Adducts: Formation and Analysis" CHEMISTRY, 59(3), 299-317.

• Chen, H.-J. C., Chen, Y.-M., Wang, T.-F., Wang, K.-S., and Shiea, J. (2001) "8-Nitroxanthine, an adduct derived from 2'-deoxyguanosine or DNA reaction with nitryl chloride." Chem. Res. Toxicol., 14, 536-546.

• Chen, H.-J. C.,* Chiang, L.C., Tseng, M.-C., Zhang, L. L., Ni, J., and Chung, F.-L. "Detection and quantification of 1,N6-ethenoadenine in human placental DNA by mass spectrometry." Chem. Res. Toxicol., 1999, 12, 1119-1126.

• Chen, H.-J. C.,* Zhang, L. L., Cox, J., Cunningham, J. A., and Chung, F.-L. "DNA Adducts of 2,3-Epoxy-4-Hydroxynonanal: Detection of 7-(1’,2-Dihydroxy-heptyl)-3H-imidazo[2,1,i]purine and 1,N⁶-Ethenoadenine by Gas Chromatography/ Negative Ion Chemical Ionization/ Mass Spectrometry." Chem. Res. Toxicol., 1998, 11(12), 1474-1480.

• Chen, H.-J. C., Applewhite, T., Jayachandran, B., and Kirk, K.L. "Synthesis of alpha-Methyl- and beta-Methyl-4,6-difluoro-5-hydroxy-tryptamine as Potential Selective Monoamine Oxidase B Inhibitors." J. Fluorine Chem., 1998, 92(1), 41-44.

• Chen, H.-J. C.,* Gonzalez, F. J., Shou, M., and Chung, F.-L. "2,3-Epoxy-4-hydroxynonanal, a potential lipid peroxidation product for etheno adduct formation, is not a substrate of human epoxide hydrolase." Carcinogenesis, 1998, 19(5), 939-943.

• Chen, H.-J. C. and Chung, F.-L. "Epoxidation of trans-4-Hydroxy-2-nonenal by Fatty Acid Hydroperoxides and Hydrogen Peroxide." Chem. Res. Toxicol., 1996, 9(1), 306-312.

• Chung, F.-L., Chen, H.-J. C., and Nath, R. "Lipid peroxidation as a potential endogenous source for the formation of exocyclic propano and etheno adducts." Carcionogenesis, 1996, 17, 2105-2111.

• Chung, F.-L., Krezeminski, J., Wang, M., Chen, H.-J. C., and Prokopczyk, B. "Formation of the Acrolein-Derived 1,N²-Propanodeoxyguanosine Adducts in DNA upon Reaction with 3-(N-Carbethoxy-N-nitrosamino)propionaldehyde", Chem. Res. Toxicol., 1994, 7(1), 62-67.

• R.G. Nath, H-J.C. Chen, R. Young-Sciame, and F.-L. Chung "A ³²P-postlabelling method for simultaneous detection and quantification of exocyclic etheno and propano adducts in DNA", Carcinogenesis, 1994, 15, 979-984.

• Chen, H.-J. C. and Chung, F.-L. "Formation of Etheno Adducts in Reaction of Enals via Autoxidation," Chem. Res. Toxicol., 1994, 7(6), 857-860.